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Laboratory diluents for research use. Not for direct human use.

Bac Water, Sterile Water or Acetic Acid Water: Which Diluent to Order

By the BacWater.fit team · Updated

Short answer. Bac water (sterile water with 0.9% benzyl alcohol) suits vials you will draw from more than once. Sterile water has no preservative: use it once, or wherever benzyl alcohol must be avoided. 0.6% acetic acid water (pH 2.7–3.1) is for the few materials whose documentation calls for a dilute acid.
Bacteriostatic water0.9% (v/v) benzyl alcohol · multi-dose · discard 28 days after first puncture · pH 5.0–7.0
Sterile waterNo preservative · single use · pH 5.0–7.0
0.6% acetic acid water0.6% (v/v) acetic acid · single use · pH 2.7–3.1

Start from the material's documentation

The datasheet or method for the material you are dissolving is the authority. If it names a diluent, use that one; if it excludes benzyl alcohol, use sterile water.

Choose bac water when

  • You will draw from the same vial over days or weeks.
  • Nothing in your method rules out benzyl alcohol.

Choose sterile water when

  • Benzyl alcohol must be avoided.
  • You use the whole vial at once.

Choose acetic acid water when

  • The documentation asks for a dilute acid, usually because the material does not dissolve at neutral pH.
  • You can use the vial in one go — it has no preservative.

Sources

  1. PubChem — Benzyl alcohol (CID 244)
  2. CDC — questions about multi-dose vials (dating and discarding opened vials)

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